Main Applications:

3d/4d cLSM, FLIM, FRAP, FRET, F(C)CS

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E02e, S2-laboratory
Image "Light Microscopy:Olympus_LSM_FluoView.jpg"

Olympus LSM FV1000

Image "Light Microscopy:BP_flowcell.jpg"

Temperature control

Sample heating and cooling via
  • Bionomic system with sample cell BC-250W and BC-110 temperature controller from 20/20 Technology, Inc.
    • Sample holder accepts standard 35 mm petri dishes
    • Control range: 0 °C - 60 °C; below 25 °C water cooling is mandatory (1 l/min)
    • Temperature accuracy: +/- 0.2 °C
    • Temperature stability: +/- 0.1 °C
    • Water cooling by F25-MC Refrigerated/Heating Circulator from Julabo
  • The objective heater system from Bioptechs is recommended for immersion objectives

Perfusion chamber

  • Homemade design consisting of thermoplastic polycarbonate to allow for transperancy and heating
  • Small channel (height: 100 µm; width: 4 mm; length: 12 mm) to guarantee laminar flow
  • Flow control (0.6 - 500 ml/h) via peristaltic pump from Pharmacia Biotech