Main Applications:

3d/4d cLSM, FLIM, FRAP, FRET, F(C)CS

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E02e, S2-laboratory
Image "Light Microscopy:Olympus_LSM_FluoView.jpg"

Olympus LSM FV1000

Image "Light Microscopy:FV1000_ports.png"

LSM unit: FluoView 1000

  • Scanning method: two galvanometer scanning mirrors
  • 3 excitation light ports:
    • 1: HBO lamp
    • 2: Olympus laser box
    • 3: Picoquant laser box
  • Image "Light Microscopy:scan_unit.jpg"Additional output port (4) connected to Avalanche photo diode detectors from Picoquant
  • UV-Vis-IR Excitation with dichroic mirror turret, 6 positions (5 DMs plus 20/80 half mirror)
  • Detection via 3 photomultiplier tubes (R7862, Hamamatsu, high sensitivity type)
    • Ch1 and Ch2 equipped with 6 position beamsplitter turret and independent grating for fast and flexible spectral detection
    • Specifications of grating units
      • Resolution: < 2 nm
      • Switching speed: 1 ms/100 nm
      • Wavelength width: 1-100 nm
    • Ch3 equipped with 6 position barrier filter turret
  • The excitation laser can be imaged on another photomultiplier tube (R7400, Hamamatsu) to obtain transmission light images (see transmission channel ChT in paragraph Filter
  • Scanning modes: X, Y, Z, t and λ, uni- or bidirectional scanning
  • Image resolution: 64 x 64 - 4096 x 4096
  • Fully motorized pinhole: displaceable in x and y-direction; diameter: 50 - 300 ┬Ám