Main Applications:

3d/4d cLSM, FLIM, FRAP, FRET, F(C)CS

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E02e, S2-laboratory
Image "Light Microscopy:Olympus_LSM_FluoView.jpg"

Olympus LSM FV1000

Filter

Microscope filter turret (No. 1)
  • Pos. 1: Mirror for LSM
  • Pos. 2: blue (U-MWU2)
    • Exciter: bandpass, 330-385 nm (BP330-385)
    • Emitter: longpass, 420 nm (BA420)
    • Beamsplitter: dichroic mirror, 400 nm (DM400)
  • Pos. 3: orange (U-MWG2)
    • Exciter: bandpass, 510-550 nm (BP510-550)
    • Emitter: longpass, 590 nm (BA590)
    • Beamsplitter: dichroic mirror, 570 nm (DM570)
  • Pos. 4: green
    • Exciter: bandpass 474-491 nm (BrightLine HC 482/18
    • Emitter: bandpass 505-545 nm (D 525/40)
    • Beamsplitter: polychroic mirror, 488 nm and 647 nm (HC-DB 488/647)
  • Pos. 5: None
  • Pos. 6: DIC Analyzer IX2-MDICT
Main dichroic beamsplitter (No. 2)
  • Pos. 1: BS 20/80
  • Pos. 2: DM 405/488
  • Pos. 3: none
  • Pos. 4: DM 405/488/559/635
  • Pos. 5: DM 458/515
  • Pos. 6: none
Secondary dichroic beamsplitter (No. 3)
  • Pos. 1: mirror
  • Pos. 2: glass
  • Pos. 3: SDM 560
  • Pos. 4: SDM 510
  • Pos. 5: SDM 490
  • Pos. 6: SDM 640
Secondary dichroic beamsplitter (No. 4)
  • Pos. 1: mirror
  • Pos. 2: glass
  • Pos. 3: SDM 640
  • Pos. 4: SDM 560
  • Pos. 5: none
  • Pos. 6: none
Barrier filter (No. 5)
  • Pos. 1: BA 655-755
  • Pos. 2: BA 575-675
  • Pos. 3: BA 575-620
  • Pos. 4-6: none
Image "Light Microscopy:FluoView_setup.png"
Filter for FLIM / F(C)CS Detection
  • Beamsplitter, 510 nm (HC-BS510)
  • Beamsplitter, 560 nm (HC-BS560)
  • Beamsplitter, 650 nm (HC-BS650)
  • Beamsplitter 50/50 (BS50/50)
  • Bandpass, 465-500 nm (BrightLine HC 482/35)
  • Bandpass, 500-530 nm (BrightLine HC 520/35)
  • Bandpass, 507-533 nm (BrightLine HC 525/15)
  • Bandpass, 500-550 nm (BrightLine HC 525/50)
  • Bandpass, 525-575 nm (BrightLine HC 550/49)
  • Bandpass, 590-625 nm (BrightLine HC 607/37)
  • Bandpass, 570-640 nm (BrightLine HC 607/70)
  • Bandpass, 600-660 nm (BrightLine HC 629/56)
  • Bandpass, 660-690 nm (BrightLine HC 676/29)
  • Bandpass, 655-725 nm (HQ690/70M, Chroma)
  • Bandpass, 670-710 nm (BrightLine HC 692/40)
  • Bandpass, 690-730 nm (BrightLine HC 710/40)
  • Longpass, 510 nm (HQ510LP)