Main Applications:

3d/4d cLSM, FLIM, FRAP, FRET, F(C)CS

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E02e, S2-laboratory
Image "Light Microscopy:Olympus_LSM_FluoView.jpg"

Olympus LSM FV1000

Fluorescence Illumination

  • external 100 W mercury fluorescence lamp with separate shutter
  • Olympus laser box with AOTF laser combiner
    • Multiline Argon: 457 nm, 488 nm, 514 nm; total power: 30 mW
    • Laser diode: 405 nm; max. power 50 mW
    • Laser diode: 559 nm; max. power 15 mW
    • Laser diode: 635 nm; max. power 20 mW
  • Picoquant picosecond pulsed laser diodes (computer-controlled via laser driver Sepia II)
    • Laser diode LDH-P-C-440B: 440 nm; pulses: < 90 ps; repitition rate: max. 40 MHz; average power @ 40MHz: 0.8 mW
    • Laser diode LDH-D-C-485: 485 nm; pulses: < 100 ps; repitition rate: max. 40 MHz; average power @ 40MHz: 0.7 mW; CW power: 50 mW
    • Laser diode LDH-D-TA-560: 560 nm; pulses: < 80 ps; repitition rate: max. 80 MHz; average power @ 80MHz: 0.5 mW; CW power: 5 mW
    • Laser diode LDH-D-C-640: 640 nm; pulses: < 100 ps; repitition rate: max. 80 MHz; average power @ 80MHz: 2.0 mW; CW power: 50 mW