Olympus TIRF 4-Line SMT

Image "Light Microscopy:BP_TIRF_4-Line_front.png"

Filter

Clean-up filter of 4-Line TIRF condenser
  • 405 nm laser: bandpass, 370 - 410 nm (BrightLine HC 390/40)
  • 488 nm laser: bandpass, 464 - 500 nm (BrightLine HC 482/18)
  • 561 nm laser: bandpass, 547 - 575 nm (BrightLine HC 561/14)
  • 640 nm laser: bandpass, 626 - 654 nm (BrightLine HC 640/14)
Microscope filter cassette
  • Pos. 1: quadcolor (blue, green, orange, red)
    • Beamsplitter: TIRF quadband polychroic mirror, 405 nm/488 nm/561 nm/640 nm (zt405/488/561/640rpc)
    • Emitter: quadbandpass, 446 nm/523 nm/600 nm/677 nm (BrightLine HC 446/523/500/677)
  • Pos. 2-5: none
  • Pos. 6: IX2-MDICT (analyzer for DIC imaging)

Image "Light Microscopy:BP_TIRF_4_Line_QV.png"Fast Emission Filter Wheel
  • Pos. 1: blue channel (DAPI, BFP): bandpass 423 - 468 nm, (BrightLine HC 445/45)
  • Pos. 2: green channel (GFP): bandpass 500 - 550 nm, (BrightLine HC 525/50)
  • Pos. 3: orange channel (TMR, mCherry): bandpass 582 - 619 nm, (BrightLine HC 600/37)
  • Pos. 4: red channel (Cy5, Atto 655): bandpass 668 - 726 nm, (BrightLine HC 697/58)
  • Pos. 5-8: empty

Quadview filter cube
  • Dichroic beamsplitter
    • 1: 480 nm, longpass (480dcxr)
    • 2: 565 nm, longpass (565dcxr)
    • 3: 640 nm, longpass (640dcxr)
  • Emission filter
    • Blue channel (DAPI, BFP): bandpass, 426 - 450 nm (BrightLine HC 438/24)
    • Green channel (GFP): bandpass, 502 - 538 nm (BrightLine HC 520/35)
    • Orange channel (TMR, mCherry): bandpass 582 - 619 nm, (BrightLine HC 600/37)
    • Red channel (Cy5, Atto 655): bandpass, 665 - 705 nm (Brightline HC 685/40)