Olympus TIRF 4-Line SMT

Image "Light Microscopy:BP_TIRF_4_Line_QV.png"

Scientific CMOS Camera

Hamamatsu ORCAFlash 4.0:
  • quantum efficiency: 70 % at 500 nm, 50 % at 750 nm
  • Peltier element plus forced-air cooled at -10 °C
  • Effective number of pixel: 2048 x 2048
  • Pixel size: 6.5 µm x 6.5 µm
  • Pixel depth: 16-bit
  • Full well capacity: 30.000 electrons
  • Readout noise: 1.3 electrons
  • Dynamic range: 23.000:1
  • Readout mode:
    • Binning readout: 2x2, 4x4
    • Sub-array readout
  • Min. exposure time
    • Free running mode: 1 ms to 10 s
    • Free running mode (sub-array): 38.96 µs to 10 s
    • External trigger mode: 1 ms to 10 s
  • Readout speed in frames per second (at center position):

    Readout speed Horizontal pixel Binning
    (2 x 2, 4 x 4)
    2048 / 1536 / 1024 / 512
    Vertical Line 2048 100
    1024 200
    512 400
    256 801
    128 1603
    64 3206
    8 25655
  • Dark current: 0.01 electrons/pixel/s (-10 °C)