Olympus TIRF 4-Line

Image "Light Microscopy:BP_TIRF_4_Line.jpg"

Microscope

Inverted research microscope Olympus IX-83 equipped with
  • motorized 8-position filter cassette
  • motorized revolving nosepiece
  • motorized xy-stage (Märzhäuser Scan IM 120x80)
  • hardware autofocus (IX3-ZDC2, 830 nm version, Olympus)
  • piezo z-stage (NanoScanZ, NZ100) from Prior Scientific
    • range of motion: 100 µm
    • repeatability: 1 nm
    • accuracy: 0.5 % of travel
    • fully software-controlled
  • long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55) with DIC prisms (60x, 100x)
  • magnification changer: 1.0x, 1.6x, 2.0x
  • white LED (Thorlabs) for transmission light with electronic shutter
  • Mid-long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55)
  • DIC imaging via:
    • polarizer U-POT (added on top of condenser)
    • Dedicated DIC prisms for 60x (IX2-MDIC60), and 100x (IX2-MDIC100) objectives
    • DIC slider U-DICTS (below revolving noisepiece)
    • DIC analyzer IX2-MDICT (located in filter turret)
  • Microscope large incubator cellVivo from Pecon:
  • Small stage top incubator for controlled CO2 concentration and 90 % humidity