Olympus TIRF 4-Line

Image "Light Microscopy:BP_TIRF_4_Line.jpg"

Filter

Microscope filter cassette
  • Pos. 1: pentacolor (blue, green, yellow/orange, red, dark red)
    • Beamsplitter: TIRF pentaband polychroic mirror, 405 nm/488 nm/561 nm/640 nm/730 nm (zt405/488/561/640/730rpc, Semrock)
    • Emitter: pentabandpass, 440 nm/521 nm/607 nm/694 nm/809 nm (BrightLine HC 440/521/607/694/809)
  • Pos. 2: Cy3 + Cy5
    • Beamsplitter: polychroic mirror, 532 nm/642 nm (zt532/642rpc)
    • Emitter: longpass at 532 nm plus notch filter at 647 nm (EdgeBasic LP 532 and zet647NF)
  • Pos. 8: IX2-MDICT (analyzer for DIC imaging)

Fast Emission Filter Wheel (Sutter Lambda 10-3)
  • Pos. 1: blue channel (DAPI, BFP): bandpass 423 - 468 nm (BrightLine HC 445/45)
  • Pos. 2: green channel (GFP): bandpass 502 - 538 nm (BrightLine HC 525/35)
  • Pos. 3: orange channel (TMR, mCherry): bandpass 575 - 625 nm (600/50 ET)
  • Pos. 4: red channel (Cy5, Atto 655): bandpass 660 - 710 nm (685/50 ET)
  • Pos. 5: far red channel (Cy7, DY-752): bandpass 768 - 849 nm (BrightLine HC 809/81)
  • Pos. 6: Dual Bandpass (GFP & mCherry): bandpass 500-550 nm, 615-700 nm(HC 527/645)
  • Pos. 7: Bypass for QuadView
  • Pos. 8-10: empty

Quadview filter cubes
Cube 1: blue, green, yellow/orange, red
  • Dichroic beamsplitter
    • 1: 480 nm, longpass (480dcxr)
    • 2: 565 nm, longpass (565dcxr)
    • 3: 640 nm, longpass (640dcxr)
  • Emission filter
    • Blue channel (DAPI, BFP): bandpass, 426 - 450 nm (BrightLine HC 438/24)
    • Green channel (GFP): bandpass, 502 - 538 nm (BrightLine HC 520/35)
    • Orange channel (TMR, mCherry): bandpass 582 - 619 nm, (BrightLine HC 600/37)
    • Red channel (Cy5, Atto 655): bandpass, 665 - 705 nm (Brightline HC 685/40)
Cube 2: green, green-orange, red, dark red
  • Dichroic beamsplitter
    • 1: 565 nm, longpass (T565LPXR)
    • 2: 630 nm, longpass (630DCXR)
    • 3: 735 nm, longpass (735DCXR)
  • Emission filter
    • Green channel (GFP, Cy2): bandpass,  502 - 538 nm (BrightLine HC 520/35)
    • Green-orange channel (TMR, Cy3): bandpass, 575 - 625 nm (ET 600/50)
    • Red channel (Atto 655, Cy5): bandpass, 660 -710 nm (ET 685/50)
    • Dark-red channel (Cy7): bandpass, 769 - 850 nm (Brightline HC 809/81)