Main Applications:

TIRF, 4-color SMT, FPALM, STORM

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E15b, S2-laboratory
Image "Light Microscopy:BP_TIRF_4_Line.jpg"

Olympus TIRF 4-Line STORM

Camera

Hamamatsu ORCA Fusion-BT
  • back-illuminated sCMOs chip
  • water cooled to -15 °C
  • quantum efficiency:
    • @ 400nm: 72%
    • @ 550nm: 95%
    • @ 700nm: 83%
    • @ 800nm: 58%
  • Effective number of pixel: 2304 x 2304
  • Pixel size: 6.5 µm x 6.5 µm
  • Pixel depth: 16-bit
  • Exposure times:
    • Fast scan: 17µs to 10s
    • Standard scan: 65µs to 10s
    • Ultra quiet scan: 280µs to 10s
  • Readout noise:
    • Fast scan: 1.6 electrons rms
    • Standard scan: 1.0 electrons rms
    • Ultra quiet scan: 0.7 electrons rms
  • Readout times at full resolution:
    • Fast scan: 11.22 ms (89.1 fps max)
    • Standard scan: 42.99 ms (23.2 fps max)
    • Ultra quiet scan: 184.4ms (5.4 fps max)
  • Pixel binning: 2x2, 4x4
  • Triggering
    • Internal
    • External: Edge, Levele, Sync Readout, Start, Global reset edge, Global reset level
    • Outputs: Global exposure timing, 3 programmable timing, Trigger ready, Low, High
    • Software trigger
  • Maximum frame rates (frames/s)
    ROI Scan mode FPS
    2304x2304 Fast 89.1
    Standard 23.2
    Ultra-quiet 5.4
    2048x2048 Fast 100
    Standard 26.1
    Ultra-quiet 6.1
    256x256 Fast 799
    Standard 208
    Ultra-quiet 48.6
  • Dark current: 0.7 electrons/pixel/s (-15 °C)
  • Dark offset: 100 counts