Olympus TIRF 4-Line

Image "Light Microscopy:BP_TIRF_4_Line.jpg"

Electron Multiplying CCD Camera

Andor iXon Ultra 897
  • Electron multiplying back-illuminated frame transfer CCD
  • forced-air cooled at -85 °C
  • quantum efficiency: >90 % between 500 - 700 nm (see  Graphs and Drawings)
  • Effective number of pixel: 512 x 512
  • Pixel size: 16 µm x 16 µm
  • Pixel depth: 16-bit
  • Pixel clock-rate:
    • EM-CCD readout: 17, 10, 5, 1 MHz
    • Normal CCD readout: 3, 1, 0.08 MHz
  • Pixel binning: 2x2, 4x4
  • Triggering
    • Internal
    • External
    • External start
    • External exposure
    • Software trigger
  • Fasted readout speed in frames per second (readout: 17 MHz, internal trigger mode)
    • Normal mode
    Array size
    Binning 512 x 512 256 x 256 128 x 128 64 x 64
    1x1 56 110 212 397
    2x2 109 210 394 699
    4x4 206 385 680 1099
    • Cropped sensor mode
    Array size
    Binning 256 x 256 128 x 128 64 x 64
    1x1 111 595 1433
    2x2 215 1085 2433
    4x4 402 1802 3577
  • Readout noise:
    • EM-CCD readout (typical):
      • at 17 MHz: 98 electrons
      • at 10 MHz: 66 electrons
      • at 5 MHz: 36 electrons
      • at 1 MHz: 15 electrons
    • EM-CCD readout (with EM):
      • at 17 MHz: <1 electrons
      • at 10 MHz: <1 electrons
      • at 5 MHz: <1 electrons
      • at 1 MHz: <1 electrons
    • Normal CCD readout:
      • at 1 MHz: 6 electrons
      • at 0.08 MHz: 3 electrons
  • Dark current: 0.001 electrons/pixel/s (-85 °C)