Olympus TIRF 4-Line

Image "Light Microscopy:BP_TIRF_4_Line.jpg"

3D Single Molecule Localization

We add cylindrical lenses between the microscope side port and the QuadView in order to generate astigmatic point spread functions. Two differnt lenses are available:
  1. focal length f=500 mm, antireflection coating (LJ1144RM-A, Thorlabs)
  2. focal length f=1000 mm, antireflection coating (LJ1516RM-A, Thorlabs)