Leica LSM SP5

LSM unit

Scanning method: two galvanometer scanning mirrors
Excitation and emission light is separated via different polychroic beamsplitters (6 position filter wheel, see Filter)
Fluorescence detection via 3 independent low-noise photomultiplier tubes
Fluorescence signal is spectrally dispersed and guided via the spectrophotometric detector to the photomultiplier tubes
A transmission detector enables bright field/DIC imaging using the scanning laser
Scanning modes: X, Y, Z, time t and wavelength λ, uni- or bidirectional scanning
Image resolution: max. 8192 x 8192
Scanning speed: up to 5 frames/s at 512 x 512 pixels
Hardware zoom: 1x, ..., 64x; continuous control