Main Applications:

3d/4d cLSM

Person in Charge:

Viktoria Liss

Location:

Building 37, Room 106, S2-laboratory
Image "Light Microscopy:MB_Leica_LSM.png"

Leica LSM SP5

Filter

  • Filter cassette for epifluorescence:
    • Blue (filter set A)
      • Exciter: bandpass, 340 - 380 nm
      • beamsplitter: dichroic mirror, 400 nm
      • Emitter: bandpass, 450 - 490 nm
    • Green (filter set I3)
      • Exciter: bandpass, 450 - 490 nm
      • beamsplitter: dichroic mirror, 510 nm
      • Emitter: longpass, 515 nm
    • Red (filter set N2.1)
      • Exciter: bandpass, 515 - 560 nm
      • beamsplitter: dichroic mirror, 580 nm
      • Emitter: longpass, 590 nm
  • Main LSM beamsplitter:
    • Pos. 1: beamsplitter with 30 % reflection, 70 % transmission (RT 30/70)
    • Pos. 2: none
    • Pos. 3: polychroic mirror, 488nm, 543 nm, 633 nm (TD 488/543/633)
    • Pos. 4: polychroic mirror, 458 nm, 514 nm (DD 458/514)
    • Pos. 5: longpass, 500 nm (RSP 500)
    • Pos. 6: polychroic mirror, 488 nm, 543 nm (DD 488/543)