Zeiss LSM 880 with fast Airyscan

Image "Light Microscopy/Zeiss LSM 880 AiryScan:IMG_2955_upload.jpg"

Filter

Filter turret for epifluorescence
  • Pos. 1: DIC Analyzer
  • Pos. 2: GFP (Zeiss filter set 38)
    • Exciter: bandpass, 450 - 490 nm
    • beamsplitter: dichroic mirror, 495 nm
    • Emitter: bandpass, 500 - 550 nm
  • Pos. 3: DsRed (Zeiss filter set 43)
    • Exciter: bandpass, 533 - 558 nm
    • beamsplitter: dichroic mirror, 570 nm
    • Emitter: bandpass, 570 - 640 nm
  • Pos. 4: CFP (Zeiss filter set 47)
    • Exciter: bandpass, 426 - 446 nm
    • beamsplitter: dichroic mirror, 455 nm
    • Emitter: bandpass, 460 - 500 nm
  • Pos. 5: DAPI (Zeiss filter set 49)
    • Exciter: bandpass, 335 - 383 nm
    • beamsplitter: dichroic mirror, 395 nm
    • Emitter: bandpass, 420 - 470 nm
Main LSM beamsplitter
  • MBS 405
  • MBS 458
  • MBS 458/514
  • MBS 458/561
  • MBS 488
  • MBS 488/561
  • MBS 488/561/633
  • MBS T80/R20
Secondary LSM beamsplitter between PMTs and Airyscan detector
  • Mirror (PMTs only)
  • SBS BP 420-460 + LP 500
  • SBS LP 525
  • SBS LP 570
  • SBS LP 660
  • SBS LP 460
  • SBS SP 615
  • Plate (Airyscan only)
Filter before Airyscan detector
  • Plate
  • BP 420-445 + BP 464-505
  • BP 420-480 + BP 495-550
  • BP 420-480 + BP 495-620
  • BP 465-505 + LP 525
  • BP 495-550 + LP 570
  • BP 570-620 + LP 645
  • free position