Zeiss LSM 510 META NLO

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Filter

Zeiss filter specifications are abbreviated with german acronyms:
  • KP = Kurzpass (shortpass)
    Example: KP 545 passes all wavelengths below 545 nm; wavelengths above 545 nm are reflected.
  • LP = Langpass (longpass)
    Example: LP 505 passes all wavelengths above 505 nm; wavelengths below 505 nm are reflected.
  • BP = Bandpass (bandpass)
    Example: BP 500-530 IR passes all wavelengths in between 500 nm and 530 nm and infrared light; all other wavelengths are reflected.
  • FT = Farbteiler (dichroic beamsplitter)
  • HFT = Hauptfarbteiler (primary dichroic beamsplitter)
    Example: HFT 458/514 deflects the indicated laser lines onto the specimen and allows the emitted fluorescent light to pass through.
  • NFT = Nebenfarbteiler (secondary dichroic beamsplitter)
    Example: NFT 490 splits the emitted fluorescence light into seperate channels. Light below 490 nm wavelength is deflected, whereas light with longer wavelengths passes through.
NFT filters are longpass filters if not further specified as KP (short pass).

Filter cassette (cube 1 on drawing)Bild "Light Microscopy:Zeiss_LSM_510_META_filters.png"
  • Pos. 1: None
  • Pos. 2: blue (Zeiss Filterset 1)
    • Exciter: bandpass, 359 - 371 nm (BP 365/12)
    • Beamsplitter: dichroic mirror, 395 nm (FT 395)
    • Emitter: longpass, 397 nm (LP 397)
  • Pos. 3: green (Zeiss Filterset 9)
    • Exciter: bandpass, 450 - 490 nm (BP 450 - 490)
    • Beamsplitter: dichroic mirror, 510 nm (FT 510)
    • Emitter: longpass, 515 nm (LP 515)
  • Pos. 4: red (Zeiss Filterset 15)
    • Exciter: bandpass, 540 - 552 nm (BP 546/12)
    • Beamsplitter: dichroic mirror, 580 nm (FT 580)
    • Emitter: longpass, 590 nm (LP 590)
  • Pos. 5: Non descanned detection filter (NDD KP685)
    • Emitter: shortpass, 685 nm (KP 685)
    • Beamsplitter: dichroic mirror, 675 nm (FT 675)
  • Pos. 6: None
Main beamsplitter (Cube 2)
  • Pos. 1: NT80/20
  • Pos. 2: HFT 488/543/633
  • Pos. 3: HFT KP 700/488 (simultaneous IR multi photon (KP 700) and single photon (488) excitation)
  • Pos. 4: HFT KP 700/543 (simultaneous IR multi photon (KP 700) and single photon (543) excitation)
  • Pos. 5: HFT 458/514
  • Pos. 6: HFT 458/543/633
  • Pos. 7: HFT 488/543
  • Pos. 8: HFT KP 650 (IR multi photon excitation)
First secondary beamsplitter (Cube 3)
  • Pos. 1: none
  • Pos. 2: mirror
  • Pos. 3: NFT 490
  • Pos. 4: NFT 515
  • Pos. 5: NFT 545
  • Pos. 6: NFT 635 Vis
  • Pos. 7: KP 545
  • Pos. 8: plate (do transmit light 100 %; set automatically)
Second secondary beamsplitter (Cube 4)
  • Pos. 1: none
  • Pos. 2: plate (do transmit light 100 %; set automatically)
  • Pos. 3: BG39 (blue green glass used to block IR excitation light)
  • Pos. 4: mirror
Third secondary beamsplitter (Cube 5)
  • Pos. 1: mirror
  • Pos. 2: NFT 490
  • Pos. 3: NFT 545
  • Pos. 4: BG39 (blue green glass used to block IR excitation light)
Emission filter for channel 2 (6)
  • Pos. 1: LP 505
  • Pos. 2: LP 560
  • Pos. 3: BP 500-530 IR
  • Pos. 4: BP 500-550 IR
  • Pos. 5: BP 535-590 IR
  • Pos. 6: BP 565-615 IR
  • Pos. 7: BP 650-710 IR
  • Pos. 8: none
Emission filter for channel 3 (7)
  • Pos. 1: KP 685
  • Pos. 2: BP 390-465 IR
  • Pos. 3: BP 435-485 IR
  • Pos. 4: BP 480-520 IR
  • Pos. 5: BP 500-530 IR
  • Pos. 6: BP 500-550 IR
  • Pos. 7: none
  • Pos. 8: Change Pos (filter can be changed manually)