Zeiss Cell Observer SD

Image "Light Microscopy:MB_Spinning_Disc.png"

Filter

Filter cassette
  • Pos. 1: Brightfield
  • Pos. 2: blue, DAPI (Zeiss Filterset 49)
    • Exciter: bandpass, 365 nm (G 365)
    • Beamsplitter: dichroic mirror, 395 nm (FT 395)
    • Emitter: bandpass 420 - 470 nm (BP 445/50)
  • Pos. 3: green, eGFP (Zeiss Filterset 38 HE)
    • Exciter: bandpass, 450 - 490 nm (BP 470/40 (HE))
    • Beamsplitter: dichroic mirror, 495 nm (FT 495 (HE))
    • Emitter: bandpass, 500 - 550 nm (BP 525/50 (HE))
  • Pos. 4: orange, Cy3 (Zeiss Filterset 43 HE)
    • Exciter: bandpass, 537 - 563 nm (BP 550/25 (HE))
    • Beamsplitter: dichroic mirror, 570 nm (FT 570 (HE))
    • Emitter: bandpass, 570 - 640 nm (BP 605/70 (HE))
  • Pos. 5: red, Cy5 (Zeiss Filterset 50)
    • Exciter: bandpass, 625 - 655 nm (BP 640/30)
    • Beamsplitter: dichroic mirror, 660 nm (FT 660)
    • Emitter: bandpass, 665 - 715 nm (BP 690/50)
  • Pos. 6: DIC TL
Spinning Disc Unit
  • Beamsplitters
    • Pos. 1: polychroic mirror, 405 nm/488 nm/568 nm/647 nm
  • Emission filter
    • bandpass, 425 - 475 nm
    • bandpass, 500 - 550 nm
    • bandpass, 598 - 660 nm
    • bandpass, 469 - 491 nm, plus longpass, 530 nm
    • dualbandpass, 500 - 554 nm and 615 - 675 nm