Zeiss Cell Observer SD
Filter
Filter cassette
- Pos. 1: Brightfield
- Pos. 2: blue, DAPI (Zeiss Filterset 49)
- Exciter: bandpass, 365 nm (G 365)
- Beamsplitter: dichroic mirror, 395 nm (FT 395)
- Emitter: bandpass 420 - 470 nm (BP 445/50)
- Pos. 3: green, eGFP (Zeiss Filterset 38 HE)
- Exciter: bandpass, 450 - 490 nm (BP 470/40 (HE))
- Beamsplitter: dichroic mirror, 495 nm (FT 495 (HE))
- Emitter: bandpass, 500 - 550 nm (BP 525/50 (HE))
- Pos. 4: orange, Cy3 (Zeiss Filterset 43 HE)
- Exciter: bandpass, 537 - 563 nm (BP 550/25 (HE))
- Beamsplitter: dichroic mirror, 570 nm (FT 570 (HE))
- Emitter: bandpass, 570 - 640 nm (BP 605/70 (HE))
- Pos. 5: red, Cy5 (Zeiss Filterset 50)
- Exciter: bandpass, 625 - 655 nm (BP 640/30)
- Beamsplitter: dichroic mirror, 660 nm (FT 660)
- Emitter: bandpass, 665 - 715 nm (BP 690/50)
- Pos. 6: DIC TL
Spinning Disc Unit
- Beamsplitters
- Pos. 1: polychroic mirror, 405 nm/488 nm/568 nm/647 nm
- Emission filter
- bandpass, 425 - 475 nm
- bandpass, 500 - 550 nm
- bandpass, 598 - 660 nm
- bandpass, 469 - 491 nm, plus longpass, 530 nm
- dualbandpass, 500 - 554 nm and 615 - 675 nm