Zeiss Cell Observer SD
Cameras
Zeiss
AxioCam MRm Rev. 3 (mounted on right camera port of the microscope)
- Monochrome, progressive-scan CCD ICX285AL from Sony
- CCD cooling via Peltier element
- quantum efficiency: >50 % between 450 - 650 nm
- Effective number of pixel: 1388 x 1040
- Pixel size: 6.45 µm x 6.45 µm
- Pixel depth: 12-bit
- Pixel clock-rate: 24.57 MHz
- Readout time: 69 ms per full frame
- Pixel binning: 2x2, 3x3, 4x4, 5x5
- Fasted readout speed in frames per second
| Binning |
H x V |
Max. frame rate |
| 1x1 |
1388 x 1040 |
14 |
| 2x2 |
692 x 520 |
26 |
| 3x3 |
460 x 344 |
35 |
| 4x4 |
344 x 260 |
43 |
| 5x5 |
272 x 208 |
50 |
- Readout noise: < 7.7 electrons
- Dark current: 0.7 electrons/pixel/s
Photometric Evolve (two versions mounted on each camera port of spinning disc unit)
- Electron multiplying back-illuminated frame transfer CCD
- Forced-air cooled at -85 °C
- Quantum efficiency: >90 % between 500 - 700 nm (see quantum efficiency curve)
- Effective number of pixel: 512 x 512
- Pixel size: 16 µm x 16 µm
- Pixel depth: 16-bit
- Pixel clock-rate:
- EM-CCD readout: 10 MHz and 5 MHz
- Normal CCD readout: 5 MHz and 1.25 MHz
- Pixel binning: 2x2, 4x4, 8x8
- Fasted readout speed in frames per second (readout: 10 MHz, exposure time: 0 s)
|
Region |
| Binning |
512x512 |
256x256 |
128x128 |
64x64 |
| 1x1 |
33.7 |
65.5 |
124 |
224 |
| 2x2 |
65.8 |
124.4 |
224 |
376 |
| 4x4 |
124 |
224 |
376 |
563 |
| 8x8 |
224 |
374.5 |
562 |
748 |
- Readout noise:
- EM-CCD readout (EM gain state 3):
- at 10 MHz: 45 electrons
- at 5 MHz: 32 electrons
- Normal CCD readout:
- at 5 MHz: 12 electrons
- at 1.25 MHz: 6 electrons
- Dark current: 0.001 electrons/pixel/s (-85 °C)