Olympus TIRF 3-Line
Filter
Filter slider of TIRF condenser for 488 nm and 561 nm laser- Pos. 1: neutral density filter, 25 % transmission
- Pos. 2: neutral density filter, 12 % transmission
- Pos. 3: neutral density filter, 6 % transmission
- Pos. 4: neutral density filter, 3 % transmission
- Slider 1: neutral density filter, 0.1 % transmission (UV-ND 3.0)
- Slider 2: neutral density filter, 1.0 % transmission (UV-ND 2.0)
- Slider 3: neutral density filter, 3.0 % transmission (UV-ND 1.5)
- Slider 4: neutral density filter, 5.0 % transmission (UV-ND 1.3)
- Slider 5: neutral density filter, 10.0% transmission (UV-ND 1.0)
- Pos. 1: green (GFP, AT488)
- Exciter: no filter, choose 488 nm laser
- Beamsplitter: dichroic mirror, 488 nm (z488rdc)
- Emitter: bandpass, 511 - 551 nm (BrightLine HC 531/40)
- Pos. 2: empty
- Pos. 3: orange (Cy3)
- Beamsplitter: dichroic mirror, 532 nm (HC BS R532)
- Emitter: bandpass, 662 - 738 nm (ET 700/75M)
- Pos. 4: quadcolor imaging (blue, green, orange, red)
- Beamsplitter:TIRF quadband polychroic mirror, 405 nm/488 nm/561 nm/640 nm (zt405/488/561/640rpc)
- Emitter: quadbandpass, 446 nm/523 nm/600 nm/677 nm (BrightLine HC 446/523/600/677)
- Pos. 5: dualcolor imaging (green, orange) plus photomanipulation with 405 nm
- Beamsplitter: polychroic mirror, 405 nm/488 nm/561 nm (zt 405/488/561rpc)
- Emitter: dualbandpass, 523 nm/610 nm (BrightLine HC 523/610)
- Pos. 6: empty
DualView filter cubesCube 1: CFP/YFP
- dichroic beamsplitter, 505 nm (505 dcxr)
- Emission filter CFP: bandpass, 450 - 480 nm (BrightLine HC 460/30)
- Emission filter YFP: bandpass, 520 - 550 nm (HQ 535/30)
- dichroic beamsplitter, 585 nm (585 dcxu)
- Emission filter green: bandpass, 500 - 550 nm (HQ 525/50)
- Emission filter orange: bandpass, 594 - 646 nm (BrightLine HC 620/52)
- dichroic beamsplitter, 640 nm (640 dcxr)
- Emission filter orange: bandpass 582 - 619 nm, (BrightLine HC 600/37)
- Emission filter red: bandpass, 655 - 725 nm (HQ 690/70)
- polarizing beamsplitter
- two polarizer