Main Applications:

2-color SMLM & SMT, TIRFM

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E15c, S2-laboratory
Image "Light Microscopy:BP_TIRF_3_Line.jpg"

Olympus TIRF 3-Line

Filter

Filter slider of TIRF condenser for 488 nm and 561 nm laser
  • Pos. 1: neutral density filter, 25 % transmission
  • Pos. 2: neutral density filter, 12 % transmission
  • Pos. 3: neutral density filter, 6 % transmission
  • Pos. 4: neutral density filter, 3 % transmission
Filter sliders of TIRF condenser for 642 nm laser
  • Slider 1: neutral density filter, 0.1 % transmission (UV-ND 3.0)
  • Slider 2: neutral density filter, 1.0 % transmission (UV-ND 2.0)
  • Slider 3: neutral density filter, 3.0 % transmission (UV-ND 1.5)
  • Slider 4: neutral density filter, 5.0 % transmission (UV-ND 1.3)
  • Slider 5: neutral density filter, 10.0% transmission (UV-ND 1.0)
Microscope filter cassette
  • Pos. 1: green
    • Exciter: no filter, choose 488 nm laser
    • Beamsplitter: dichroic mirror, 488 nm (z488rdc)
    • Emitter: bandpass, 511 - 551 nm (BrightLine HC 531/40)
  • Pos. 2: dualcolor (green-yellow, red)
    • Exciter: dualbandpass, 527 nm/645 nm (HC 527/645)
    • Beamsplitter: polychroic mirror, 532 nm/642 nm (zt532/642rpc)
    • Emitter: longpass, 532 nm; notchfilter, 635 nm (Edge Basic 532 LP, zet635nf)
  • Pos. 3:
    • Beamsplitter: dichroic mirror, 532 nm (HC BS R532)
    • Emitter: bandpass, 662 - 738 nm (ET 700/75M)
  • Pos. 4: quadcolor imaging (blue, green, orange, red)
    • Beamsplitter:TIRF quadband polychroic mirror, 405 nm/488 nm/561 nm/640 nm (zt405/488/561/640rpc)
    • Emitter: quadbandpass, 446 nm/523 nm/600 nm/677 nm (BrightLine HC 446/523/600/677)
  • Pos. 5: dualcolor imaging (green, orange) plus photomanipulation with 405 nm
    • Beamsplitter: polychroic mirror, 405 nm/488 nm/561 nm (zt 405/488/561rpc)
    • Emitter: dualbandpass, 523 nm/610 nm (BrightLine HC 523/610)
  • Pos. 6: orange
    • Beamsplitter: dichroic mirror, 561 nm (BrightLine HC BS R561)
    • Emitter: bandpass, 589 - 625 nm (BrightLine HC 607/36)

Image "Light Microscopy:BP_TIRF_3_Line_dualview.png"DualView filter cubes
Cube 1: CFP/YFP
  • dichroic beamsplitter, 505 nm (505 dcxr)
  • Emission filter CFP: bandpass, 450 - 480 nm (BrightLine HC 460/30)
  • Emission filter YFP: bandpass, 520 - 550 nm (HQ 535/30)
Cube 2: green/orange
  • dichroic beamsplitter, 585 nm (585 dcxu)
  • Emission filter green: bandpass, 500 - 550 nm (HQ 525/50)
  • Emission filter orange: bandpass, 594 - 646 nm (BrightLine HC 620/52)
Cube 3: orange/red
  • dichroic beamsplitter, 640 nm (640 dcxr)
  • Emission filter orange:  bandpass 582 - 619 nm, (BrightLine HC 600/37)
  • Emission filter red: bandpass, 655 - 725 nm (HQ 690/70)
Cube 4: fluorescence anisotropy imaging
  • polarizing beamsplitter
  • two polarizer