Main Applications:

2-color SMLM & SMT, TIRFM

Person in Charge:

Rainer Kurre

Location:

Building 38, Room E15c, S2-laboratory
Image "Light Microscopy:BP_TIRF_3_Line.jpg"

Olympus TIRF 3-Line

Electron Multiplying CCD Camera

Andor iXon3 897
  • Electron multiplying back-illuminated frame transfer CCD
  • forced-air cooled at -85 °C
  • quantum efficiency: >90 % between 500 - 700 nm.
  • Effective number of pixel: 512 x 512
  • Pixel size: 16 µm x 16 µm
  • Pixel depth: True 14-bit
  • Pixel clock-rate:
    • EM-CCD readout: 10, 5, 3, 1 MHz
    • Normal CCD readout: 3, 1 MHz
  • Pixel binning: 2x2, 4x4, 8x8
  • Triggering
    • Internal
    • External
    • External start
    • External exposure
    • Software trigger
  • Fasted readout speed in frames per second (readout: 10 MHz, internal trigger mode)
    • Normal mode
    Array size
    Binning 512 x 512 256 x 256 128 x 128 64 x 64
    1x1 35 68 132 251
    2x2 68 132 248 452
    4x4 131 246 439 735
    8x8 231 426 699 1031
    • Cropped sensor mode
    Array size
    Binning 256 x 256 128 x 128 64 x 64
    1x1 69 395 988
    2x2 136 743 1764
    4x4 260 1327 2902
    8x8 483 2184 4285
  • Readout noise:
    • EM-CCD readout (typical):
      • at 10 MHz: 49 electrons
      • at 5 MHz: 42 electrons
      • at 3 MHz: 32 electrons
      • at 1 MHz: 21 electrons
    • EM-CCD readout (with EM):
      • at 17 MHz: <1 electrons
      • at 10 MHz: <1 electrons
      • at 5 MHz: <1 electrons
      • at 1 MHz: <1 electrons
    • Normal CCD readout:
      • at 1 MHz: 6 electrons
  • Dark current: 0.001 electrons/pixel/s (-85 °C)