Main Applications:

NLO imaging and photomanipulation, 3d/4d cLSM, widefield epi-fluorescence imaging

Person in Charge:

Rainer Kurre, Laura Vittadello

Location:

Building 38, Room E04, S2-laboratory
Image "Light Microscopy/Olympus LSM FLUOVIEW 3000 NLO:IMG_2984.jpg"

Olympus LSM FV3000 NLO

Filter

Microscope filter turret
  • Pos. 1: Mirror for LSM
  • Pos. 2: DIC Analyzer IX3-FDICT
  • Pos. 3: DAPI/FITC/TRITC/Cy5
    • Beamsplitter: 405 nm/493 nm/561 nm/647 nm (89402bs, Chroma)
    • Emitter: quadband, 420-450 nm, 505-530 nm, 578-610 nm, 665-725 nm (89402m, Chroma)
  • Pos. 4: CYF/YFP/mCherry
    • Beamsplitter: 459 nm/526 nm/596 nm (FF459/526/596-Di01, Semrock)
    • Emitter: tripleband, 461-487 nm, 529-555 nm, 578-610 nm, 600-800 nm (FF01-475/543/702-25, Semrock)
  • Pos. 5: BFP/GFP/mCherry
    • Beamsplitter: 409 nm/493 nm/596 nm (FF409/493/596-Di01, Semrock)
    • Emitter: tripleband, 412-452 nm, 498-548 nm, 600-800 nm (FF01-432/523/702-25, Semrock)
Microscope second deck (bread board position)
  • Cube 1: Multiphoton beamsplitter
    • Beamsplitter: multiphoton 700-1500 nm (T700spxr-1500, Chroma)
    • Emitter: shortpass 720 nm (HC720SP, Semrock)
  • Cube 2: DAPI/FITC/TRITC/Cy5/IR
    • Beamsplitter: 405 nm/488 nm/561 nm/642 nm/730-1200 nm (special design (laser), Chroma)
Main dichroic beamsplitter (LSM)
  • Pos. 1: DM 405/488/561/642
  • Pos. 2: DM 405/488
  • Pos. 3: DM 405/488/561
  • Pos. 4: DM 405/488/561/640/730-1200
  • Pos. 5: DM 445/514/561
  • Pos. 6: DM 445/488/561/635rpc
  • Pos. 7: MPE-SP 800
Secondary dichroic beamsplitter (LSM)
  • Pos. 1: mirror
  • Pos. 2: glass
  • Pos. 3: SDM 400-470
  • Pos. 4: SDM 400-495
  • Pos. 5: SDM 400-540
  • Pos. 6: SDM 400-580
  • Pos. 6: SDM 400-620

Fast filter wheel (camera)
  • Pos. 1: DAPI/BFP, 432/36 BrightLine HC (Semrock)
  • Pos. 2: CFP, 482/25 BrightLine HC (Semrock)
  • Pos. 3: GFP, 515/30 BrightLine HC (Semrock)
  • Pos. 4: YFP, 544/24 BrightLine HC (Semrock)
  • Pos. 5: mCherry, 595/31 BrightLine HC (Semrock)
  • Pos. 6: Cy5, 698/70 BrightLine HC (Semrock)
  • Pos. 7: GFP/DsRed Dual, 512/630 BrightLine HC (Semrock)
  • Pos. 8: Empty