Main Applications:

Multicolor 3d/4d volumetric imaging, SMLM

Person in Charge:

Rainer Kurre, Michael Holtmannspötter

Location:

Building 38, Room E15d, S2-laboratory
Image "Light Microscopy:LatticeScope.jpg"

Lattice Light-Sheet Microscope

sCMOS Camera

Hamamatsu ORCAFlash 4.0 V2
  • quantum efficiency (V2): 72 % at 560 nm, 50 % at 750 nm
  • Peltier element plus water cooled at -10 °C
  • Effective number of pixel: 2048 x 2048
  • Pixel size: 6.5 µm x 6.5 µm
  • Pixel depth: 16-bit
  • Full well capacity: 30.000 electrons
  • Readout noise: 1.3 electrons
  • Dynamic range: 23.000:1
  • Readout mode:
    • Binning readout: 2x2, 4x4
    • Sub-array readout
  • Min. exposure time
    • Free running mode: 1 ms to 10 s
    • Free running mode (sub-array): 38.96 µs to 10 s
    • External trigger mode: 1 ms to 10 s
  • Readout speed in frames per second (at center position):

    Readout speed Horizontal pixel Binning
    (2 x 2, 4 x 4)
    2048 / 1536 / 1024 / 512
    Vertical Line 2048 100
    1024 200
    512 400
    256 801
    128 1603
    64 3206
    8 25655

Inspection cameras