Olympus TIRF 1-Line with JPK AFM

Electron Multiplying CCD Camera

Andor iXon3 897

Electron multiplying back-illuminated frame transfer CCD
forced-air cooled at -85 °C
quantum efficiency: >90 % between 500 - 700 nm.
Effective number of pixel: 512 x 512
Pixel size: 16 µm x 16 µm
Pixel depth: True 14-bit
Pixel clock-rate:
- EM-CCD readout: 10, 5, 3, 1 MHz
- Normal CCD readout: 3, 1 MHz
Pixel binning: 2x2, 4x4, 8x8
Triggering
- Internal
- External
- External start
- External exposure
- Software trigger
Fasted readout speed in frames per second (readout: 10 MHz, internal trigger mode)
- Normal mode
Array size

Binning 512 x 512 256 x 256 128 x 128 64 x 64

1x1 35 68 132 251

2x2 68 132 248 452

4x4 131 246 439 735

8x8 231 426 699 1031
- Cropped sensor mode
Array size

Binning 256 x 256 128 x 128 64 x 64

1x1 69 395 988

2x2 136 743 1764

4x4 260 1327 2902

8x8 483 2184 4285
Readout noise:
- EM-CCD readout (typical):
  - at 10 MHz: 49 electrons
  - at 5 MHz: 42 electrons
  - at 3 MHz: 32 electrons
  - at 1 MHz: 21 electrons
- EM-CCD readout (with EM):
  - at 17 MHz: <1 electrons
  - at 10 MHz: <1 electrons
  - at 5 MHz: <1 electrons
  - at 1 MHz: <1 electrons
- Normal CCD readout:
  - at 1 MHz: 6 electrons
Dark current: 0.001 electrons/pixel/s (-85 °C)