Main Applications:

Flow cytometry and FACS

Person in Charge: Christian Schröer, Malte Kellermann, Elisabeth Suedhoff, Antje Möhlmeyer, Wladislaw Kohl

Location:

Building 38/E08a (S2 lab) (CellNanOs)
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Sony FACS SH800S

Overview

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform.

Specifications

  • FACS analyser and sorter
  • Lasers:
    • 405 nm (BFP, Hoechst, DyeCycle Violet)
    • 488 nm (FITC, AF488, PI, GFP, PE, YFP, Per CP, Cybr. Green, Syto 9)
    • 561 nm (mCherry, TexasRed, mRFP)
    • 640 nm (APC, Cy5, Af647)
  • Two filtersets with 8 optical parameters per set: FSC, SSC, 6 fluorescence parameters
  • Microfludic sorting chips (70 µm, 100 µm, 130 µm)
  • Exchangeable sample lines
  • Index sorting into 96- or 384-well plates
  • 2-way sorting into FACS tubes and 15 ml Falcons

Software

  • Cell Sorter Software (Sony)

Accessories

Aerosol Management System to sort BSL2 material.

Website